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Catalog Number Size Price
LS-C347438-0.2 0.2 mg $598 
Antibody

Monoclonal Mouse Anti‑Human IgM Secondary Antibody (clone NI 179, HRP, IHC) LS‑C347438

Monoclonal Mouse Anti‑Human IgM Secondary Antibody (clone NI 179, HRP, IHC) LS‑C347438

Antibody:
Human IgM Mouse anti-Human Monoclonal (HRP) (NI 179) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Price
Catalog Number
$598
LS-C347438-0.2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Human IgM Mouse anti-Human Monoclonal (HRP) (NI 179) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Other formats:

Specifications

Description
Human IgM antibody LS-C347438 is an HRP-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC.
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
NI 179
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with FITC, TRITC, Biotin.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Highly purified monoclonal IgM isolated from pooled human serum.
Specificity
Human IgM, subclass specific.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The reactivity of the antiserum is restricted to an isotype specific determinant of the IgM molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:250 upwards. in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/25/2024