Human IgG

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NI 335 and NI 343

Horseradish Peroxidase. Also available Unconjugated or conjugated with TRITC, FITC, Biotin.

Purified IgG

Unmodified

Highly purified polyclonal IgG isolated from human serum.

Human IgG, isotype specific.

• IHC
• IHC - Paraffin
• ICC
• Immunofluorescence
• ELISA
• Dot Blot
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins. To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500. in ELISA from 1:1000 upwards. in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Lyophilized from PBS, pH 7.2, No preservatives added

Reconstitute with distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.