(tested or 100% immunogen sequence identity)
- IHC (1:500 - 1:1000)
- Western blot (1:2000 - 1:10000)
- ELISA (1:10000 - 1:100000)
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Human IgG antibody was raised against human IgG (gamma chain).
Recognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. No reaction was observed against human IgM, IgA, kappa or lambda proteins. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.
Suitable for Western Blot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Western Blot: 1:2000-1:10000. ELISA: 1:10000-1:100000. Immunohistochemistry: 1:500-1:1000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized, PBS, pH 7.2, 10 mg/ml BSA (protease, IgG free), 0.01% Gentamicin sulfate
1,000 µl Sterile ddH2O
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.