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Antibodies > MLH1 > LS-C407936
 
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Catalog Number Size Price
LS-C407936-10 10 µg $318 
LS-C407936-100 100 µg $470 
MLH1 Antibody - Western blot analysis of MLH1 using anti-MLH1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85KD. The expected band size for MLH1 is at 85KD.
MLH1 Antibody - MLH1 antibody Western blot. All lanes: Anti MLH1 at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD. Observed band size: 84 kD.
MLH1 Antibody - Western blot analysis of MLH1 using anti-MLH1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85KD. The expected band size for MLH1 is at 85KD.
MLH1 Antibody - MLH1 antibody Western blot. All lanes: Anti MLH1 at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Mouse Testis Tissue Lysate at 50 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD. Observed band size: 84 kD.
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Polyclonal Rabbit anti‑Human MLH1 Antibody (aa722‑756, WB) LS‑C407936

Polyclonal Rabbit anti‑Human MLH1 Antibody (aa722‑756, WB) LS‑C407936

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Antibody:
MLH1 Rabbit anti-Human Polyclonal (aa722-756) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407936-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MLH1 Rabbit anti-Human Polyclonal (aa722-756) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MLH1 antibody LS-C407936 is an unconjugated rabbit polyclonal antibody to MLH1 (aa722-756) from human. It is reactive with human and mouse. Validated for WB.
Target
Human MLH1
Synonyms
MLH1 | COCA2 | FCC2 | HMLH1 | HNPCC | HNPCC2 | MutL protein homolog 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human MLH1 (722-756 aa KALRSHILPPKHFTEDGNILQLANLPDLYKVFERC), different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
Epitope
aa722-756
Specificity
Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MLH1
P40692 NM_000249 NP_000240.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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