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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3475-50 | 50 µl | $440 |
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IHC‑plus™ Monoclonal Mouse anti‑Human MLH1 Antibody (clone 4C9C7, IHC, IF, WB) LS‑B3475
IHC‑plus™ Monoclonal Mouse anti‑Human MLH1 Antibody (clone 4C9C7, IHC, IF, WB) LS‑B3475
Note: This antibody replaces LS-C102880
Antibody:
MLH1 Mouse anti-Human Monoclonal (4C9C7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MLH1 Mouse anti-Human Monoclonal (4C9C7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
MLH1 antibody LS-B3475 is an unconjugated mouse monoclonal antibody to human MLH1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MLH1
Synonyms
MLH1 | COCA2 | FCC2 | HMLH1 | HNPCC | HNPCC2 | MutL protein homolog 1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
4C9C7
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.\
Specificity
Human, Monkey
Applications
- IHC
- IHC - Paraffin (1:200)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3475 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MLH1
Validation
Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ MLH1 Antibody (clone 4C9C7) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3475 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Rat, Hamster
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human
Applications:
IHC - Paraffin, IHC - Frozen
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
