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MLH1 Antibody (aa387‑403, clone 164C819) LS‑C566
Note: This antibody replaces LS-C72709
MLH1 antibody LS-C566 is an unconjugated mouse monoclonal antibody to MLH1 from human and monkey. Validated for WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen
![Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/mlh1-antibody-clone-m1-ls-c133324/51224_964693.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Rat, Hamster
Applications:
IHC, IHC - Paraffin
Product Description
MLH1 antibody LS-C566 is an unconjugated mouse monoclonal antibody to MLH1 from human and monkey. Validated for WB.
Specifications
Target
Human MLH1
Synonyms
MLH1, COCA2, FCC2, HMLH1, HNPCC, HNPCC2, MutL protein homolog 1
Host
Mouse
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
[164C819]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
- Western blot (2 - 3 µg/ml)
Immunogen
MLH1 antibody was raised against this antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 387-403 DSREQKLDAFLQPLSKP of human MLH1; GenBank no. NP_000240.1. This peptide sequence is 93% homologous to mouse and dog MLH1. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Gibbon (94%); Elephant, Panda, Bat, Dog, Rabbit (88%); Mouse, Hamster, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus (82%).
Epitope
aa387-403
Specificity
In Molt-4, a 83 kD band is observed.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About MLH1
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.
P40692
NM_000249
NP_000240.1
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Images
Western blot
Western blot of MLH1 in Molt-4 cell lysate using antibody at 2 ug/ml, and exposed for 15 minutes.
Western blot
Western blot of MLH1 in Molt-4 cell lysate using antibody at 2 ug/ml, and exposed for 15 minutes.
Western blot
Western blot of MLH1 in Molt-4 cell lysate using antibody at 2 ug/ml, and exposed for 15 minutes.
Western blot
Western blot of MLH1 in Molt-4 cell lysate using antibody at 2 ug/ml, and exposed for 15 minutes.
Requested From: United States
Date Requested: 10/19/2018
Date Requested: 10/19/2018
