(tested or 100% immunogen sequence identity)
Ion exchange chromatography
Rat thymocyte membrane glycoproteins.
Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. The antibody cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. It is particularly useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4.