Rat MHC Class II RT1.Bu
(tested or 100% immunogen sequence identity)
Rat thymocyte membrane glycoproteins.
Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.4, 0.09% Sodium Azide, 1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.