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Antibodies > METTL3 > LS-C756516
 
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Catalog Number Size Price
LS-C756516-10 10 µg $318 
LS-C756516-100 100 µg $470 
METTL3 Antibody - ICC analysis of METTL3 using anti-METTL3 antibody. METTL3 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-METTL3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
METTL3 Antibody - Western blot analysis of METTL3 using anti-METTL3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysate,Lane 3: human K562 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for METTL3 at approximately 64KD. The expected band size for METTL3 is at 64KD.
METTL3 Antibody - ICC analysis of METTL3 using anti-METTL3 antibody. METTL3 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-METTL3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
METTL3 Antibody - Western blot analysis of METTL3 using anti-METTL3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human HepG2 whole cell lysate,Lane 3: human K562 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: human Caco-2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for METTL3 at approximately 64KD. The expected band size for METTL3 is at 64KD.
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Polyclonal Rabbit anti‑Human METTL3 Antibody (WB) LS‑C756516

Polyclonal Rabbit anti‑Human METTL3 Antibody (WB) LS‑C756516

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Antibody:
METTL3 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756516-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
METTL3 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
METTL3 antibody LS-C756516 is an unconjugated rabbit polyclonal antibody to METTL3 from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human METTL3
Synonyms
METTL3 | M6A | Methyltransferase like 3 | MTA70 | Spo8 | IME4 | MRNA m(6)A methyltransferase | MT-A70
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human METTL3 recombinant protein (Position: Q423-D571).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About METTL3
Q86U44 AF014837 AAB71850.1

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Featured Products

METTL3 Antibody - Detection of Human METTL3/MT-A70 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-METTL3/MT-A70 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
METTL3 Antibody (aa1‑50) LS‑C288359
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
METTL3 Antibody - Immunohistochemical analysis of METTL3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
METTL3 Antibody LS‑C483015
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
METTL3 Antibody - METTL3 antibody Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
METTL3 Antibody (aa503‑552) LS‑C107543
Species: Human
Applications: Western blot
METTL3 Antibody - METTL3 antibody Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
METTL3 Antibody (C‑Terminus, FITC) LS‑C431439
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
METTL3 Protein - 12.5% SDS-PAGE of human METTL3 stained with Coomassie Blue
Human METTL3 Protein (Recombinant GST) (Full Length) - LS-G34553
Source: Wheat
Tag: GST
BPIFA1 / SPLUNC1 ELISA Kit
Human BPIFA1 / SPLUNC1 (Sandwich ELISA) ELISA Kit - LS-F50015
Reactivity: Human
Range: 40-10000 pg/ml
See all METTL3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/6/2024
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