MARK2 | ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:250 - 1:1000)
- ELISA (1:1000 - 1:3000)
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This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kD corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.