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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C18889-100 100 µg (1 mg/ml) $425 
MARK2 Antibody - Western Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane. Primary antibody: MARK2 pT595 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

Polyclonal MARK2 Antibody (phospho‑Thr595, IHC, IF, WB) LS‑C18889

Polyclonal MARK2 Antibody (phospho‑Thr595, IHC, IF, WB) LS‑C18889

Antibody:
Rabbit Polyclonal to Human MARK2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18889-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MARK2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
MARK2 antibody LS-C18889 is an unconjugated rabbit polyclonal antibody to MARK2 from human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human MARK2
Synonyms
MARK2 | ELKL motif kinase 1 | EMK-1 | ELKL motif kinase | EMK1 | PAR-1 | PAR1 homolog | Par1b | Par-1b | Ser/Thr protein kinase PAR-1B | Emk | PAR1 homolog b
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:250 - 1:1000)
  • ELISA (1:1000 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a.  The immunogen peptide was phosphorylated at the residue corresponding to T595.
Epitope
pThr595
Specificity
Reactivity occurs against human MARK2 pT595 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4. A BLAST analysis was used to suggest reactivity with this protein from human, dog, bovine, mouse and rat sources based on 100% homology for the immunogen sequence. Cross reactivity with MARK2 homologues from other sources has not been determined.
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kD corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MARK2
Q7KZI7 NM_017490 NP_059672.2

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Requested From: United States
Date Requested: 2/22/2020