Polyclonal Rabbit anti‑Human MAPKAPK5 / PRAK Antibody (phospho‑Thr182, IHC, IF, WB) LS‑C800215
Polyclonal Rabbit anti‑Human MAPKAPK5 / PRAK Antibody (phospho‑Thr182, IHC, IF, WB) LS‑C800215
Antibody:
MAPKAPK5 / PRAK Rabbit anti-Human Polyclonal (pThr182) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Overview
Antibody:
MAPKAPK5 / PRAK Rabbit anti-Human Polyclonal (pThr182) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
PRAK antibody LS-C800215 is an unconjugated rabbit polyclonal antibody to PRAK (MAPKAPK5) (pThr182) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human MAPKAPK5 / PRAK
Synonyms
MAPKAPK5 | MAPKAP kinase 5 | MAPKAP-K5 | MK5 | p38-regulated/activated kinase | MAPKAPK-5 | PRAK | MK-5 | p38-regulated protein kinase
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Threonine 182.
Specificity
Phospho-MAPKAPK5(Thr182) Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Threonine 182.
Applications
- IHC (1:50 - 1:200)
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, Western blot, ELISA
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse
Applications:
IHC, Western blot
Reactivity:
Human, Mouse
Range:
Positive/Negative
Reactivity:
Human, Mouse
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024