LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

MAPK8 / JNK1 Antibody LS-C17753

Antibody

MAPK8 / JNK1 Antibody LS-C17753

Antibody:
Rabbit Polyclonal to Rat MAPK8 / JNK1
Application:
WB, IP, Func
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$605 
LS-C17753-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rat MAPK8 / JNK1
Application:
WB, IP, Func
Reactivity:
Rat, Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
JNK1 antibody LS-C17753 is an unconjugated rabbit polyclonal antibody to JNK1 (MAPK8) from human, mouse and rat. Validated for Func, IP and WB.

Target
Rat MAPK8 / JNK1
Host
Rabbit
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
MAPK8 / JNK1 antibody was raised against gST fusion protein corresponding to full-length rat SAPK1a. This sequence is approximately 85% identical to rat SAPK1b and rat SAPK1c. These sequences are highly conserved among mammalian species.
Specificity
Recognizes SAPK1a (54kD). Weakly recognizes SAPK1b (54kD). The protein detected with a MWapp of 45kD may be SAPK1c. Species Reactivity: Human, mouse and rat.
Usage
Suitable for use in Western Blot analysis and Immunoprecipitation. Western Blot Analysis: 1 ug/ml detected SAPK1 in RIPA lysates from human A431, murine 3T3 and rat PC-12 cells. At 1 ug/ml, the signal detected with 10 ng of gst-SAPK1a was approximately three times greater than the signal detected of with 10 ng gst-SAPK1b. Included Positive Antigen Control: Non-stimulated A431 cell lysate. Use 20 ug per lane for minigels. Immunoprecipitation Protein Kinase Assay: 4 ug immunoprecipitated active recombinant rat SAPK1a, which then catalyzed the transfer of the y-phosphate of [y-32 P]-ATP to c-Jun protein substrate. Western Blot Analysis A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SAPK1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.01 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK8 / JNK1
P45983 NM_139049 NP_620637.1

Publications (0)

Reviews (0)

Featured Products

Reactivity: Mouse
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 27.43-20000 pg/ml
Reactivity: Mouse
Range: 7.8-500 pg/ml
Reactivity: Human
Range: 78-5000 pg/ml

Requested From: United States
Date Requested: 7/15/2019
Get Social With Us!
Follow us on Facebook Follow us on LinkedIn
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy