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LIN28A / LIN28 Antibody (phospho‑Ser134) LS‑C163637

Catalog Number Size Price
LS-C163637-400 400 µl $325 
Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
Dot blot of anti-Phospho-LIN28-S134 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
Dot blot of anti-Phospho-LIN28-S134 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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LIN28A / LIN28 Antibody (phospho‑Ser134) LS‑C163637

Antibody:
Rabbit Polyclonal to Human LIN28A / LIN28
Application:
ICC, IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C163637-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human LIN28A / LIN28
Application:
ICC, IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LIN28 antibody LS-C163637 is an unconjugated rabbit polyclonal antibody to human LIN28 (LIN28A ). Validated for DB, ICC and IF.
Target
Human LIN28A / LIN28
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (1:100)
  • Dot Blot (1:500)
Blocking Peptide
LS-E7473 - Lyophilized - 100 µg - $230.00
Immunizing peptide used to generate LS-C163637. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
pSer134
Specificity
This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIN28A / LIN28
Q9H9Z2 NM_024674 NP_078950.1

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Anti-LIN28A / LIN28 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, Western blot
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry

Requested From: United States
Date Requested: 8/24/2019
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