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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C163637-400 400 µl $325 
LIN28A / LIN28 Antibody - Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
LIN28A / LIN28 Antibody - Dot blot of anti-Phospho-LIN28-S134 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
LIN28A / LIN28 Antibody - Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
LIN28A / LIN28 Antibody - Dot blot of anti-Phospho-LIN28-S134 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Polyclonal Rabbit anti‑Human LIN28A / LIN28 Antibody (phospho‑Ser134, IF) LS‑C163637

Polyclonal Rabbit anti‑Human LIN28A / LIN28 Antibody (phospho‑Ser134, IF) LS‑C163637

Antibody:
Rabbit Polyclonal to Human LIN28A / LIN28
Application:
ICC, IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C163637-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human LIN28A / LIN28
Application:
ICC, IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LIN28 antibody LS-C163637 is an unconjugated rabbit polyclonal antibody to human LIN28 (LIN28A) (pSer134). Validated for DB, ICC and IF.
Target
Human LIN28A / LIN28
Synonyms
LIN28A | CSDD1 | Lin-28 homolog (C. elegans) | LIN-28 | Lin-28A | LIN28 | Protein lin-28 homolog A | Lin-28 homolog A (C. elegans) | ZCCHC1 | RNA-binding protein LIN-28
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence (1:100)
  • Dot Blot (1:500)
Blocking Peptide
LS-E7473 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C163637. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
pSer134
Specificity
This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIN28A / LIN28
Q9H9Z2 NM_024674 NP_078950.1

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Requested From: United States
Date Requested: 6/1/2020