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Catalog Number Size Price
LS-B11566-200 200 µl $460 
LIN28A / LIN28 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
LIN28A / LIN28 Antibody - Fluorescent confocal image of SY5Y cells stained LIN28A antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated LIN28A primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
LIN28A / LIN28 Antibody - Immunofluorescence of A549 cells, using LIN28A Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
LIN28A / LIN28 Antibody - LIN28A Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The LIN28A antibody detected the LIN28A protein (arrow).
LIN28A / LIN28 Antibody - LIN28A Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LIN28A / LIN28 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
LIN28A / LIN28 Antibody - Fluorescent confocal image of SY5Y cells stained LIN28A antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated LIN28A primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
LIN28A / LIN28 Antibody - Immunofluorescence of A549 cells, using LIN28A Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
LIN28A / LIN28 Antibody - LIN28A Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The LIN28A antibody detected the LIN28A protein (arrow).
LIN28A / LIN28 Antibody - LIN28A Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human LIN28A / LIN28 Antibody (aa108‑138, IHC, IF, WB) LS‑B11566

IHC‑plus™ Polyclonal Rabbit anti‑Human LIN28A / LIN28 Antibody (aa108‑138, IHC, IF, WB) LS‑B11566

Note: This antibody replaces LS-C165782
Antibody:
LIN28A / LIN28 Rabbit anti-Human Polyclonal (aa108-138) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11566-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LIN28A / LIN28 Rabbit anti-Human Polyclonal (aa108-138) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
LIN28 antibody LS-B11566 is an unconjugated rabbit polyclonal antibody to LIN28 (LIN28A) (aa108-138) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human LIN28A / LIN28
Synonyms
LIN28A | CSDD1 | Lin-28 homolog (C. elegans) | LIN-28 | Lin-28A | LIN28 | Protein lin-28 homolog A | Lin-28 homolog A (C. elegans) | ZCCHC1 | RNA-binding protein LIN-28
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa108-138
Specificity
This LIN28A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human LIN28A.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LIN28A / LIN28
Q9H9Z2 NM_024674 NP_078950.1

Validation

LIN28A / LIN28 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ LIN28A / LIN28 Antibody (aa108-138) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B11566 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

A Rapid Induction Mechanism for Lin28a in Trophic Responses. Alexandra M Amen, Claudia R Ruiz-Garzon, Jay Shi, Megha Subramanian, Daniel L Pham, Mollie K Meffert. Molecular cell. 2017 Feb;65:490-503e7.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/24/2024