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Antibodies > LAMC1 / Laminin Gamma 1 > LS-C662196
 
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Catalog Number Size Price
LS-C662196-10 10 µg $318 
LS-C662196-100 100 µg $470 
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse heart tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - Western blot analysis of Laminin using anti-Laminin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysate,Lane 2: rat cardiac muscle tissue lysate,Lane 3: rat lung tissue lysate,Lane 4: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Laminin at approximately 200KD. The expected band size for Laminin is at 177KD.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse heart tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - IHC analysis of Laminin using anti-Laminin antibody. Laminin was detected in paraffin-embedded section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Laminin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LAMC1 / Laminin Gamma 1 Antibody - Western blot analysis of Laminin using anti-Laminin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysate,Lane 2: rat cardiac muscle tissue lysate,Lane 3: rat lung tissue lysate,Lane 4: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Laminin at approximately 200KD. The expected band size for Laminin is at 177KD.
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Polyclonal Rabbit anti‑Rabbit LAMC1 / Laminin Gamma 1 Antibody (IHC, WB) LS‑C662196

Polyclonal Rabbit anti‑Rabbit LAMC1 / Laminin Gamma 1 Antibody (IHC, WB) LS‑C662196

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Antibody:
LAMC1 / Laminin Gamma 1 Rabbit anti-Rabbit Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662196-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
LAMC1 / Laminin Gamma 1 Rabbit anti-Rabbit Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Laminin Gamma 1 antibody LS-C662196 is an unconjugated rabbit polyclonal antibody to rabbit Laminin Gamma 1 (LAMC1). Validated for IHC and WB.
Target
Rabbit LAMC1 / Laminin Gamma 1
Synonyms
LAMC1 | Laminin subunit gamma-1 | Laminin-7 subunit gamma | Laminin-8 subunit gamma | Laminin-1 subunit gamma | Laminin-4 subunit gamma | Laminin-9 subunit gamma | Laminin B2 chain | Laminin gamma-1 chain | Laminin-3 subunit gamma | Laminin-6 subunit gamma | S-laminin subunit gamma | Laminin Gamma 1 | Laminin-10 subunit gamma | Laminin-11 subunit gamma | Laminin-2 subunit gamma | S-LAM gamma
Host
Rabbit
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
Peptide mixture of laminin gamma1,2,3(NKLNEIEGSLNKAKDEMKAS; DLEERVRRQRNHLHLLETSI; LQLDSHGALHHKLRQLEEES). Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.
Specificity
Found in the basement membranes (major component).
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAMC1 / Laminin Gamma 1
P11047 NM_002293 NP_002284.3

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LAMC1 / Laminin Gamma 1 Antibody (clone 2E6‑B4) LS‑C139064
Species: Human
Applications: Western blot, ELISA
LAMC1 / Laminin Gamma 1 Antibody - Anti-LAMC1 / Laminin Gamma 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
PathPlus™ LAMC1 / Laminin Gamma 1 Antibody (aa526‑1106) LS‑B7354
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
LAMC1 / Laminin Gamma 1 Antibody
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Species: Human
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LAMC1 / Laminin Gamma 1 Antibody - Western blot of Laminin gamma-1 antibody
LAMC1 / Laminin Gamma 1 Antibody (1420‑1500 aa, C‑terminal) LS‑C384320
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
LAMC1 / Laminin Gamma 1 Antibody - LAMC1 / Laminin Gamma 1 antibody. IHC(P): Human Intestinal Cancer Tissue.
LAMC1 / Laminin Gamma 1 Antibody (aa1566‑1579) LS‑C312737
Species: Human, Monkey, Rat, Horse
Applications: IHC, IHC - Paraffin, Western blot
LAMC1 / Laminin Gamma 1 Antibody - Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
LAMC1 / Laminin Gamma 1 Antibody (C‑Terminus) LS‑C356151
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
See all LAMC1 / Laminin Gamma 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/10/2024
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