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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAMC1 / Laminin Gamma 1 Antibody (C‑Terminus) LS‑C356151
Laminin Gamma 1 antibody LS-C356151 is an unconjugated rabbit polyclonal antibody to Laminin Gamma 1 (LAMC1) from human and monkey. Validated for IHC and WB.
Catalog
Size
Price
LS-C356151-100
100 µl (1 mg/ml)
$245

Popular LAMC1 / Laminin Gamma 1 Products

Detection limit for recombinant GST tagged LAMC1 is approximately 0.3 ng/ml as a capture antibody.
Species: Human
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Anti-LAMC1 / Laminin Gamma 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Western blot Image
Species: Human
Applications: Western blot, ELISA
Western blot of Laminin gamma-1 antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Laminin Gamma 1 antibody LS-C356151 is an unconjugated rabbit polyclonal antibody to Laminin Gamma 1 (LAMC1) from human and monkey. Validated for IHC and WB.

Specifications

Target
Human LAMC1 / Laminin Gamma 1
Synonyms
LAMC1, Laminin subunit gamma-1, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-1 subunit gamma, Laminin-4 subunit gamma, Laminin-9 subunit gamma, Laminin B2 chain, Laminin gamma-1 chain, Laminin-3 subunit gamma, Laminin-6 subunit gamma, S-laminin subunit gamma, Laminin Gamma 1, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, S-LAM gamma
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Laminin gamma 1.
Blocking Peptide
LS-E41715 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C356151. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Laminin gamma 1 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
About LAMC1 / Laminin Gamma 1
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. P11047 NM_002293 NP_002284.3


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Images

Immunohistochemistry

Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.
Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.
Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.
Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.

Immunohistochemistry

Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Laminin gamma 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Western blot

Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.
Western blot analysis of Laminin gamma 1 expression in PC3 (A); COS7 (B) whole cell lysates.

Requested From: United States
Date Requested: 11/13/2018

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