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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C391583-100 100 µg (1 mg/ml) $465 
KRT19 / CK19 / Cytokeratin 19 Antibody - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 19 antibody (A53-B/A2.26 + BA17).  This image was taken for the unmodified form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human KRT19 / CK19 / Cytokeratin 19 Antibody (clone A53‑B/A2.26 + BA17, Azide‑free, IHC, IF) LS‑C391583

Monoclonal Mouse anti‑Human KRT19 / CK19 / Cytokeratin 19 Antibody (clone A53‑B/A2.26 + BA17, Azide‑free, IHC, IF) LS‑C391583

Antibody:
KRT19 / CK19 / Cytokeratin 19 Mouse anti-Human Monoclonal (Azide-free) (A53-B/A2.26 + BA17) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:
Price
Catalog Number
$465
LS-C391583-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
KRT19 / CK19 / Cytokeratin 19 Mouse anti-Human Monoclonal (Azide-free) (A53-B/A2.26 + BA17) Antibody
Application:
IHC, IHC-P, IF, Flo
Reactivity:
Human
Format:
Unconjugated, Azide-free
Other formats:

Specifications

Description
Cytokeratin 19 antibody LS-C391583 is an unconjugated mouse monoclonal antibody to human Cytokeratin 19 (KRT19 / CK19). Validated for Flow, IF and IHC.
Target
Human KRT19 / CK19 / Cytokeratin 19
Synonyms
KRT19 | CK19 | Cytokeratin 19 | Cytokeratin-19 | Keratin 19 | Keratin-19 | CK-19 | K19 | K1CS | Keratin, type I, 40-kd
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 + IgG2a Monoclonal
Clone
A53-B/A2.26 + BA17
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Immunogen
MCF-7 cells (A53-B/A2) and human mammary epithelial organoids (BA17) were used as the immunogen for this Cytokeratin 19 antibody cocktail.
Specificity
Human KRT19 / CK19 / Cytokeratin 19
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. The optimal dilution of the Cytokeratin 19 antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT19 / CK19 / Cytokeratin 19
P08727 NM_002276 NP_002267.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/24/2021