Target
Human KRT19 / CK19 / Cytokeratin 19
Synonyms
KRT19 | CK19 | Cytokeratin 19 | Cytokeratin-19 | Keratin 19 | Keratin-19 | CK-19 | K19 | K1CS | Keratin, type I, 40-kd
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Cytokeratin 19 of Mr 40000; from cultured human MCF-7 cells. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (88%); Mouse, Horse (82%).
Specificity
Antigen Recognized in Human, rat, fish, bovine; Species (tested so far) not with mouse, woodchuck, chicken, xenopus. Reactivities on Cultured Cell Lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Polypeptide Reacting: Mr 40 000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa352-368 (VRADSERQNQEYQRLMD) of the a-helical fragment.
Applications
- IHC
- IHC - Frozen
- Western blot
- ELISA
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Usage
Suitable for Immunohistochemistry (frozen tissue), Western Blot (Western) and ELISA. Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT.
Reconstitution
Reconstitute in deionized water
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT19 / CK19 / Cytokeratin 19