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Catalog Number Size Price
LS-C104667-50 50 µg $356 
LS-C104667-100 100 µg $396 
KITLG / SCF Antibody - Western Blot (reducing) of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KITLG / SCF Antibody - Western Blot (non-reducing) of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KITLG / SCF Antibody - Sandwich ELISA of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KITLG / SCF Antibody - Western Blot (reducing) of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KITLG / SCF Antibody - Western Blot (non-reducing) of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KITLG / SCF Antibody - Sandwich ELISA of SCF antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Human KITLG / SCF Antibody (WB) LS‑C104667

Polyclonal Rabbit anti‑Human KITLG / SCF Antibody (WB) LS‑C104667

Note: This antibody replaces LS-C54267, LS-C17771
Antibody:
KITLG / SCF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$356
LS-C104667-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KITLG / SCF Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
SCF antibody LS-C104667 is an unconjugated rabbit polyclonal antibody to human SCF (KITLG). Validated for ELISA and WB.
Target
Human KITLG / SCF
Synonyms
KITLG | C-Kit ligand | KIT ligand | Kitl | KL-1 | SHEP7 | SF | Steel factor | Stem cell factor | SCF | FPH2 | Mast cell growth factor | MGF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human SCF.
Specificity
Human SCF
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 0.5 ug/ml-1 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with enzyme induced antigen retrieval (proteinase K) at room temperature. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (10.00 ng/ml), a concentration of 0.64-0.96 ug/ml of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104672) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KITLG / SCF
P21583 NM_000899 NP_000890.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/23/2024