Target
Human IRF4
Synonyms
IRF4 | Interferon regulatory factor 4 | NF-EM5 | LSIRF | IRF-4 | Multiple myeloma oncogene 1
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Synthetic peptide corresponding to residues around Asp175 of human IRF-4 (KLH-coupled).
Specificity
Recognizes endogenous levels of human total IRF-4 protein. Species cross-reactivity: rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:100)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100. Chromatin IP: 1:100.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. For long-term storage and to avoid freeze-thaw cycles, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for up to 6 months at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee