IRF4 antibody LS-C80182 is an unconjugated rabbit polyclonal antibody to IRF4 from human, mouse, rat and other species. Validated for IHC and WB.
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with HRP
IRF4 antibody was raised against synthetic peptide from N-Terminus of human IRF4 (Q15306, NP_002451). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Horse, Pig, Opossum, Guinea pig, Xenopus (100%); Dog, Rabbit, Turkey, Zebra finch, Chicken, Salmon (92%); Stickleback (90%); Zebrafish (85%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.5 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.