(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:100)
IRF4 antibody was raised against synthetic peptide corresponding to residues around Asp175 of human IRF-4 (KLH-coupled).
Recognizes endogenous levels of human total IRF-4 protein. Species cross-reactivity: rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100. Chromatin IP: 1:100.
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Short term: store at 4°C. For long-term storage and to avoid freeze-thaw cycles, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for up to 6 months at -20°C.
For research use only.