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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF4 Antibody (C‑Terminus) LS‑C110245
IRF4 antibody LS-C110245 is an unconjugated rabbit polyclonal antibody to IRF4 from human, chicken, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C110245-50
50 µg
$365
Description
IRF4 antibody LS-C110245 is an unconjugated rabbit polyclonal antibody to IRF4 from human, chicken, dog and other species. Validated for WB.
Target
Human IRF4
Host
Rabbit
Reactivity
Human, Dog, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
IRF4 antibody was raised against synthetic peptide from C-Terminus of human IRF4 (Q15306, NP_002451). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Dog, Turkey, Zebra finch, Chicken (100%); Galago, Marmoset, Elephant, Panda, Bat, Horse, Guinea pig (92%); Opossum (91%); Rabbit (85%); Pig (83%).
Blocking Peptide
LS-E13701 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110245. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human IRF4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About IRF4
Q15306 NM_002460 NP_002451.2

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Western blot of IRF4 (arrow) using rabbit polyclonal IRF4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.
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Images

Western blot

IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF4 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/18/2018
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