IRF4 antibody LS-C110245 is an unconjugated rabbit polyclonal antibody to IRF4 from human, chicken, dog and other species. Validated for WB.
Human, Dog, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
IRF4 antibody was raised against synthetic peptide from C-Terminus of human IRF4 (Q15306, NP_002451). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Dog, Turkey, Zebra finch, Chicken (100%); Galago, Marmoset, Elephant, Panda, Bat, Horse, Guinea pig (92%); Opossum (91%); Rabbit (85%); Pig (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110245. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.