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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF4 Antibody (C‑Terminus) IHC‑plus™ LS‑B7104
Note: This antibody replaces LS-C144371
IRF4 antibody LS-B7104 is an unconjugated rabbit polyclonal antibody to human IRF4. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7104-50
50 µg (1 mg/ml)
Unavailable

Popular IRF4 Products

IRF4 antibody LS-C80182 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-IRF-4 antibody IHC of human neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4362 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Rat
Applications: Western blot, Immunoprecipitation
Western blot of IRF4 (arrow) using rabbit polyclonal IRF4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.
Species: Human
Applications: Western blot, Flow Cytometry

Product Description

IRF4 antibody LS-B7104 is an unconjugated rabbit polyclonal antibody to human IRF4. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About IRF4
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Q15306 NM_002460 NP_002451.2

IRF4 Antibody, Interferon regulatory factor 4 Antibody, NF-EM5 Antibody, LSIRF Antibody, IRF-4 Antibody, Multiple myeloma oncogene 1 Antibody


Specifications

Target
Human IRF4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E31117 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B7104. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
IRF4 antibody is predicted to not cross-react with other IRF protein family members. IRF4 often migrates at a higher than predicted molecular weight in SDS-PAGE.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.
Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.

Western blot

Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.

Western blot

Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.
Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.

Western blot

Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.

Western blot

Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.
Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.

Western blot

Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.

Western blot

Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.
Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.
Immunocytochemistry of IRF4 in Jurkat cells with IRF4 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.
Immunofluorescence of IRF4 in Jurkat cells with IRF4 antibody at 20 ug/ml.

Western blot

Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.
Western blot analysis of IRF4 in Ramos cell lysate with IRF4 antibody at 0.5 µg/mL.

Western blot

Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of IRF4 in Jurkat cell lysate with IRF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Requested From: 
Date Requested: 7/18/2018

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