LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IRF4 Antibody (aa433‑445) LS‑C154961
IRF4 antibody LS-C154961 is an unconjugated goat polyclonal antibody to human IRF4. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C154961-100
100 µg (0.5 mg/ml)
Unavailable

Popular IRF4 Products

IRF4 antibody LS-C80182 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Horse, Pig, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-IRF-4 antibody IHC of human neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4362 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Rat
Applications: Western blot, Immunoprecipitation
Anti-IRF-4 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7104 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of IRF4 (arrow) using rabbit polyclonal IRF4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRF4 gene.
Species: Human
Applications: Western blot, Flow Cytometry

Product Description

IRF4 antibody LS-C154961 is an unconjugated goat polyclonal antibody to human IRF4. Validated for Peptide-ELISA and WB.
About IRF4
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Q15306 NM_002460 NP_002451.2

IRF4 Antibody, Interferon regulatory factor 4 Antibody, NF-EM5 Antibody, LSIRF Antibody, IRF-4 Antibody, Multiple myeloma oncogene 1 Antibody


Specifications

Target
Human IRF4
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Immunogen
IRF4 antibody was raised against synthetic peptide C-HISNPEDYHRSIR from the C-terminus of human IRF-4 (NP_002451.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Galago, Marmoset, Elephant, Panda, Horse (92%); Bat, Dog, Rabbit, Guinea pig (85%).
Blocking Peptide
LS-E27831 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154961. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa433-445
Specificity
Human IRF-4.
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 48kD band observed in lysates of cell lines Daudi and Jurkat and of Human Skin (calculated MW of 51.8kD according to NP_002451.2). Recommended concentration: 0.5-2 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images

Western blot

IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF4 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 7/16/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number