(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:50)
- Western blot (1:1000)
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IRF3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to a region around residue 300 of IRF3.
Detects endogenous levels of human IRF3 at 51kD mouse and rat, at 57kD human. Species cross-reactivity: mouse, rat and monkey.
Immunohistochemistry: LS-B2529 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2529 was determined to be 1:50.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.