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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF3 Antibody (phospho‑Ser385) LS‑C199138
IRF3 antibody LS-C199138 is an unconjugated rabbit polyclonal antibody to human IRF3. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199138-50
50 µl (1 mg/ml)
$265
LS-C199138-100
100 µl (1 mg/ml)
$325

Popular IRF3 Products

Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunofluorescent staining using IRF3 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-IRF3 antibody.
Species: Human
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot
Anti-IRF3 Antibody (phospho-Ser396) antibody at 1:500 dilution with Jurkat cells treated with 200 ng/ml of EGF for 30 minutes.
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Anti-IRF3 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

IRF3 antibody LS-C199138 is an unconjugated rabbit polyclonal antibody to human IRF3. Validated for IF, IHC, Peptide-ELISA and WB.

Specifications

Target
Human IRF3
Synonyms
IRF3, Interferon regulatory factor 3, IRF-3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IRF3 antibody was raised against synthetic peptide derived from human IRF-3 around the phosphorylation site of Ser385. (AA Range: 351-400)
Epitope
pSer385
Specificity
IRF-3 (Phospho-Ser385) Antibody detects endogenous levels of IRF-3 only when phosphorylated at serine385.
Usage
Western Blot: Predicted MW 47 kDa
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
About IRF3
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Q14653 NM_001571 NP_001562.1


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Images

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot

Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HT29 cells treated with INSULIN 0.01U/ML 15', using IRF-3 (Phospho-Ser385) Antibody. The lane on the right is blocked with the phospho peptide.

Requested From: United States
Date Requested: 11/16/2018

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