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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF3 Antibody (clone 1522CT766.58.24) LS‑C392581
IRF3 antibody LS-C392581 is an unconjugated mouse monoclonal antibody to IRF3 from human and african green monkey. Validated for Flow, IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C392581-50
50 µl
Unavailable
LS-C392581-200
200 µl
Unavailable

Popular IRF3 Products

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Antibody
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Anti-IRF3 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2529 dilution 1:50.
Species: Human, Monkey
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Immunofluorescent staining using IRF3 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-IRF3 antibody.
Species: Human
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot
Anti-IRF3 Antibody (phospho-Ser396) antibody at 1:500 dilution with Jurkat cells treated with 200 ng/ml of EGF for 30 minutes.
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

IRF3 antibody LS-C392581 is an unconjugated mouse monoclonal antibody to IRF3 from human and african green monkey. Validated for Flow, IF, IHC, Peptide-ELISA and WB.
About IRF3
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Q14653 NM_001571 NP_001562.1

IRF3 Antibody, Interferon regulatory factor 3 Antibody, IRF-3 Antibody


Specifications

Target
Human IRF3
Host
Mouse
Reactivity
Human, African green monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1522CT766.58.24]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Immunofluorescence (1:25)
  • Western blot (1:2000)
  • Flow Cytometry (1:25)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% sodium azide.
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescence

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Western blot

All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow Cytometry

Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemistry

Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescence

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Western blot

All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow Cytometry

Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemistry

Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescence

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Western blot

All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow Cytometry

Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Immunohistochemistry

Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Antibody staining IRF3 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescence

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1:25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).

Western blot

All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IRF3 Antibody at 1:2000 dilution. Lane 1: COS-7 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow Cytometry

Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Jurkat cells stained with antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (antibody, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed) at 1:400 dilution for 40 min at 37 ° C. Isotype control antibody (blue line) was mouse IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Requested From: 
Date Requested: 7/20/2018

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