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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154092-100 100 µg (1 mg/ml) $425 
IL7 Antibody - Anti-IL-7 Antibody - Western Blot. anti-Human IL-7 antibody shows detection of a band ~17 kD in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

IL7 Antibody LS‑C154092

IL7 Antibody LS‑C154092

Antibody:
Rabbit Polyclonal to Human IL7
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154092-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human IL7
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL7 antibody LS-C154092 is an unconjugated rabbit polyclonal antibody to human IL7. Validated for ELISA and WB.
Target
Human IL7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Specificity
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 17.4 kD in size corresponding to native human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL7
P13232 NM_000880 NP_000871.1

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Requested From: United States
Date Requested: 11/15/2019