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Catalog Number Size Price
LS-C153806-100 100 µg (1 mg/ml) $552 
IL7 Antibody - anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Human IL7 Antibody (HRP, IHC, WB) LS‑C153806

Polyclonal Rabbit anti‑Human IL7 Antibody (HRP, IHC, WB) LS‑C153806

Antibody:
IL7 Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$552
LS-C153806-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL7 Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
IL7 antibody LS-C153806 is an HRP-conjugated rabbit polyclonal antibody to human IL7. Validated for ELISA, IHC and WB.
Target
Human IL7
Synonyms
IL7 | IL-7 | Interleukin-7 | Interleukin 7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
Specificity
This antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.4 kD in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL7
P13232 NM_000880 NP_000871.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/9/2024