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Antibodies > IL7 > LS-C312626
 
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Catalog Number Size Price
LS-C312626-10 10 µg $318 
LS-C312626-100 100 µg (0.5 mg/ml) $470 
IL7 Antibody - IL7 antibody. IHC(P): Mouse Spleen Tissue.
IL7 Antibody - Western blot analysis of IL7 using anti-IL7 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD.
IL7 Antibody - WB of IL7 antibody. All lanes: Anti-IL7 at 0.5ug/ml. Lane 1: Recombinant Mouse IL-7 Protein 10ng. Lane 2: Recombinant Mouse IL-7 Protein 5ng. Lane 3: Recombinant Mouse IL-7 Protein 2.5ng. Predicted bind size: 15KD. Observed bind size: 15KD.
IL7 Antibody - IL7 antibody. IHC(P): Mouse Spleen Tissue.
IL7 Antibody - Western blot analysis of IL7 using anti-IL7 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD.
IL7 Antibody - WB of IL7 antibody. All lanes: Anti-IL7 at 0.5ug/ml. Lane 1: Recombinant Mouse IL-7 Protein 10ng. Lane 2: Recombinant Mouse IL-7 Protein 5ng. Lane 3: Recombinant Mouse IL-7 Protein 2.5ng. Predicted bind size: 15KD. Observed bind size: 15KD.
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Polyclonal Rabbit anti‑Mouse IL7 Antibody (aa26‑42, IHC, WB) LS‑C312626

Polyclonal Rabbit anti‑Mouse IL7 Antibody (aa26‑42, IHC, WB) LS‑C312626

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Note: This antibody replaces LS-C388665
Antibody:
IL7 Rabbit anti-Mouse Polyclonal (aa26-42) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312626-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL7 Rabbit anti-Mouse Polyclonal (aa26-42) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL7 antibody LS-C312626 is an unconjugated rabbit polyclonal antibody to mouse IL7 (aa26-42). Validated for IHC and WB.
Target
Mouse IL7
Synonyms
IL7 | IL-7 | Interleukin-7 | Interleukin 7
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of mouse IL7(26-42 aa ECHIKDKEGKAYESVLM).
Epitope
aa26-42
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL7
P13232 NM_000880 NP_000871.1

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Species: Mouse
Applications: Western blot, ELISA, Functional Assay, Neutralization
IL7 Antibody - Western Blot (reducing) of Interleukin 7 / IL-7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody LS‑C104554
Species: Human
Applications: Western blot, ELISA
IL7 Antibody - anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
IL7 Antibody (HRP) LS‑C153806
Species: Human
Applications: IHC, Western blot, ELISA
IL7 Antibody - Biotin Rabbit Anti-Human IL-7 Antibody - Western Blot. Western Blot showing detection of Human IL-7. 50 ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated - 209-406-B94 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 17 kD molecular weight position expected for Human IL-7. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody (Biotin) LS‑C154121
Species: Human
Applications: IHC, Western blot, ELISA
IL7 Antibody - Western Blot (reducing) of Interleukin 7 / IL-7 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL7 Antibody LS‑C104812
Species: Mouse
Applications: Western blot, ELISA
IL7 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ IL7 Antibody LS‑B14351
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
See all IL7 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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