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Catalog Number Size Price
LS-C745223-25 25 µl (1 mg/ml) $275 
IL6 / Interleukin 6 Antibody - Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
IL6 / Interleukin 6 Antibody - Anti-Human IL-6 Antibody - Flow Cytometry Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
IL6 / Interleukin 6 Antibody - Flow Cytometry of Human anti-IL-6 antibody 209-301-310 Cells: human PBMC Stimulation: Figure A: un-stimulated, Figure B: 1 µg/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6
IL6 / Interleukin 6 Antibody - Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Human IL-6 (MOUSE) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.
IL6 / Interleukin 6 Antibody - Anti-Human IL-6 Antibody - Flow Cytometry Human PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours. Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly. 1mL of permeabilization buffer was added. 0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes. 1:100 of strep/PE was added and incubated for 30 minutes. LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.
IL6 / Interleukin 6 Antibody - Flow Cytometry of Human anti-IL-6 antibody 209-301-310 Cells: human PBMC Stimulation: Figure A: un-stimulated, Figure B: 1 µg/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6
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Monoclonal Mouse anti‑Human IL6 / Interleukin 6 Antibody (clone 33A12.G9, WB) LS‑C745223

Monoclonal Mouse anti‑Human IL6 / Interleukin 6 Antibody (clone 33A12.G9, WB) LS‑C745223

Antibody:
IL6 / Interleukin 6 Mouse anti-Human Monoclonal (33A12.G9) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745223-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL6 / Interleukin 6 Mouse anti-Human Monoclonal (33A12.G9) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Interleukin 6 antibody LS-C745223 is an unconjugated mouse monoclonal antibody to human Interleukin 6 (IL6). Validated for ELISA, Flow and WB.
Target
Human IL6 / Interleukin 6
Synonyms
IL6 | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
33A12.G9
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
IL-6 monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E.coli followed by hybridoma development.
Applications
  • Western blot (1:1000)
  • Flow Cytometry (0.5 mg/ml)
  • ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6 / Interleukin 6
P05231 NM_000600 NP_000591.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021