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Catalog Number Size Price
LS-B7482-50 50 µg $440 
IL6 / Interleukin 6 Antibody - Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL6 / Interleukin 6 Antibody - Western Blot (non-reducing) of IL-6 antibody
IL6 / Interleukin 6 Antibody - Sandwich ELISA of IL-6 antibody
IL6 / Interleukin 6 Antibody - Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL6 / Interleukin 6 Antibody - Western Blot (non-reducing) of IL-6 antibody
IL6 / Interleukin 6 Antibody - Sandwich ELISA of IL-6 antibody
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PathPlus™ Polyclonal Rabbit anti‑Human IL6 / Interleukin 6 Antibody (IHC, WB) LS‑B7482

PathPlus™ Polyclonal Rabbit anti‑Human IL6 / Interleukin 6 Antibody (IHC, WB) LS‑B7482

Note: This antibody replaces LS-C104566, LS-C42362
Antibody:
IL6 / Interleukin 6 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$440
LS-B7482-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL6 / Interleukin 6 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IL6 (Interleukin 6) is both an anti-inflammatory myokine and a pro-inflammatory cytokine involved in inflammation, the immune response and body temperature control. It functions in the inflammatory response to microbial pathogens in the innate immune system. IL6 is involved in regulating fever and it can cross the blood-brain barrier to change the temperature setpoint by activating PGE2 synthesis in the hypothalamus. It is also involved in osteoclast formation after being secreted by osteoblasts, and it stimulates the production of neutrophils in bone marrow. It may be positively involved in the consolidation and processing of emotional memories during sleep, and is also thought to be involved in psychological stress. In cancer and diseases, it promotes inflammatory and auto-immune processes in Alzheimer’s, arthritis, atherosclerosis, diabetes, depression and in various cancers. In immunohistochemistry, IL6 has variable secretory positivity in tissues throughout the body. References: Neuroscience Letters. 179 (1–2): 53–6, PMID: 7845624; FASEB Journal. 23 (10): 3629–36, PMID: 19546306; Psychosomatic Medicine. 61 (2): 175–80, PMID: 10204970; Diabetes. 54 Suppl 2: S114–24, PMID: 16306329; Cytokine & Growth Factor Reviews. 12 (1): 33–40, PMID: 11312117; Biological Psychiatry. 67 (5): 446–57, PMID: 20015486;
Target
Human IL6 / Interleukin 6
Synonyms
IL6 | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human IL-6.
Specificity
Human IL-6
Applications
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B7482 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-6 (1 ng/ml), a concentration of 0.20-0.40 ug/ml of this antibody is required. ELISA: To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 (LS-C104546) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute in sterile water to 0.1-1 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6 / Interleukin 6
P05231 NM_000600 NP_000591.1

Validation

IL6 / Interleukin 6 Antibody - Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-6 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ IL6 / Interleukin 6 Antibody for IHC, WB/Western, ELISA LS-B7482 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Fetal skin as a pro-inflammatory organ: Evidence from a primate model of chorioamnionitis. Boonkasidecha S, Kannan PS, Kallapur SG, Jobe AH, Kemp MW. PloS one. 2017 September;12:e0184938. (Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/22/2021