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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154077-100 100 µg (1 mg/ml) $425 
IL36RN / IL1F5 Antibody - Anti-Bovine IL-1F5 Antibody - Western Blot. Western blot of Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kD (arrowhead) raised in yeast. The identity of the band at 36 kD is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG ( for 45 min at room temperature.

Polyclonal IL36RN / IL1F5 Antibody (Lyophilized, WB) LS‑C154077

Polyclonal IL36RN / IL1F5 Antibody (Lyophilized, WB) LS‑C154077

Antibody:
Rabbit Polyclonal to Bovine IL36RN / IL1F5
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$425 
LS-C154077-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Bovine IL36RN / IL1F5
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized

Specifications

Description
IL1F5 antibody LS-C154077 is an unconjugated rabbit polyclonal antibody to bovine IL1F5 (IL36RN ). Validated for ELISA and WB.
Target
Bovine IL36RN / IL1F5
Synonyms
IL36RN | FIL1 | FIL1D | IL-1 delta | IL-1 related protein 3 | IL-1HY1 | IL-1RP3 | IL-1-related protein 3 | IL1F5 | IL1RP3 | IL1L1 | IL36RA | Interleukin-1 delta | Family of interleukin 1-delta | FIL1 delta | IL-1L1 | IL-1ra homolog 1 | Interleukin-1 family member 5 | Interleukin-1-like protein 1 | FIL1(DELTA) | IL-1F5 | IL1HY1 | Interleukin-1 HY1 | PSORP
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:8000)
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
Specificity
This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog, panda, and ferret based on 90-92% homology with the immunizing sequence.
Usage
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kD in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL36RN / IL1F5
Q9UBH0 NM_012275 NP_036407.1

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Requested From: United States
Date Requested: 2/25/2020