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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IL36RN / IL1F5 Antibody LS‑C154077

Catalog Number Size Price
LS-C154077-100 100 µg (1 mg/ml) $425 
Anti-Bovine IL-1F5 Antibody - Western Blot. Western blot of Protein-A Purified anti-bovine IL-1F5 antibody shows detection of recombinant bovine IL-1F5 at 17.1 kD (arrowhead) raised in yeast. The identity of the band at 36 kD is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 ug/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG ( for 45 min at room temperature.

IL36RN / IL1F5 Antibody LS‑C154077

Antibody:
Rabbit Polyclonal to Bovine IL36RN / IL1F5
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154077-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Bovine IL36RN / IL1F5
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
IL1F5 antibody LS-C154077 is an unconjugated rabbit polyclonal antibody to bovine IL1F5 (IL36RN ). Validated for ELISA and WB.
Target
Bovine IL36RN / IL1F5
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • ELISA
Immunogen
Recombinant protein produced in yeast, corresponding to the 155 amino acids of the mature bovine IL-1F5 protein.
Specificity
This antibody is specific for bovine IL-1F5 protein. A BLAST analysis was used to suggest cross-reactivity with IL-1F5 from dog and mouse sources based on 94% homology with the immunizing sequence. Partial reactivity is expected against horse based on 91% homology, and to human, chimpanzee, macaque, and rat based on 90% homology with the immunizing sequence.
Usage
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17.1 kD in size corresponding to bovine IL-1F5 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL36RN / IL1F5
Q9UBH0 NM_012275 NP_036407.1

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Featured Products

Product
Species: Human
Applications: IHC, Western blot, ELISA
Reactivity: Human
Range: 15.63-1000 pg/ml
Reactivity: Rat
Range: 15.625-1000 pg/ml

Requested From: United States
Date Requested: 8/19/2019
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