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Catalog Number Size Price
LS-C662003-10 10 µg $318 
LS-C662003-100 100 µg $470 
IL1RN Antibody - Western blot analysis of IL1RA using anti-IL1RA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human COLO-320 whole cell lysate,Lane 3: human HepG2 whole cell lysate,Lane 4: human Jurkat whole cell lysate,Lane 5: human K562 whole cell lysate,Lane 6: human HT1080 whole cell lysate,Lane 7: human U20S whole cell lysate,Lane 8: human PANC-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA at approximately 23KD. The expected band size for IL1RA is at 20KD.

Polyclonal Rabbit anti‑Human IL1RN Antibody (WB) LS‑C662003

Polyclonal Rabbit anti‑Human IL1RN Antibody (WB) LS‑C662003

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662003-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL1RN antibody LS-C662003 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA and WB.
Target
Human IL1RN
Synonyms
IL1RN | DIRA | IL-1ra | IL-1RN | IL1F3 | IL1RA | MVCD4 | IL1 inhibitor | IL1RN (IL1F3) | IRAP | ICIL-1RA | IL-1ra3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IL1RA recombinant protein (Position: R26-E177). Human IL1RA shares 77% and 75.5% amino acid (aa) sequence identity with mouse and rat IL1RA, respectively.
Specificity
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1RN
P18510 NM_000577 NP_000568.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024