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Catalog Number Size Price
LS-C745232-100 100 µg (1 mg/ml) $567 
Antibody

Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑C745232

Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑C745232

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C745232-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL1RN antibody LS-C745232 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, Flow, IHC, Neut, RIA and WB.
Target
Human IL1RN
Synonyms
IL1RN | DIRA | IL-1ra | IL-1RN | IL1F3 | IL1RA | MVCD4 | IL1 inhibitor | IL1RN (IL1F3) | IRAP | ICIL-1RA | IL-1ra3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
DEAE chromatography
Modifications
Unmodified
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.
Specificity
This antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes. This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry. When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly.
Applications
  • IHC (1:400)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:1000 - 1:5000)
  • Neutralization
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1RN
P18510 NM_000577 NP_000568.1

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Requested From: United States
Date Requested: 11/6/2024