Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-15 (5 ng/ml), a concentration of 0.4-0.5 ug/ml of this antibody is required. ELISA: To detect mIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-15 (LS-C104416
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-15. Western Blot: To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.