Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/ml-1 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-15 (1 ng/ml), a concentration of 0.15-0.20 ug/ml of this antibody is required. ELISA: To detect hIL-15 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-15 (LS-C104326
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.