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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL10RB Antibody (aa87‑243, HRP) LS‑C710039
IL10RB antibody LS-C710039 is an HRP-conjugated rabbit polyclonal antibody to human IL10RB. Validated for IHC and WB.
Catalog
Size
Price
LS-C710039-20
20 µl
$215
LS-C710039-100
100 µl
$285
LS-C710039-200
200 µl
$335
LS-C710039-1
1 ml
$650
LS-C710039-10
10 ml
$2,580

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Species: Human
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Immunohistochemistry image at a dilution of 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Western blot of recombinant IL10RB / IL-10R2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot

Product Description

IL10RB Antibody (aa87-243, HRP) for IHC, WB/Western LS-C710039

Specifications

Target
Human IL10RB
Synonyms
IL10RB, CDW210B, CDw210b antigen, D21S66, IL-10R subunit 2, IBD25, IL-10R2, Interleukin 10 receptor, beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, CRF2-4, CRFB4, D21S58
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC, FITC, Cy3, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL10RB antibody was raised against recombinant IL10Rb (Leu87-Ala243) expressed in E. coli.
Epitope
aa87-243
Specificity
The antibody is a rabbit polyclonal antibody raised against IL10Rb. It has been selected for its ability to recognize IL10Rb in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About IL10RB
Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Q08334 NM_000628 NP_000619.3


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Images

Western blot

Western blot of recombinant IL10RB / IL-10R2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant IL10RB / IL-10R2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant IL10RB / IL-10R2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant IL10RB / IL-10R2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant IL10RB / IL-10R2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant IL10RB / IL-10R2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant IL10RB / IL-10R2.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant IL10RB / IL-10R2. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/19/2018

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