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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B6311-200 200 µl $425 
Tuberin / TSC2 Antibody - Anti-TSC2 / Tuberin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Tuberin / TSC2 Antibody - Fluorescent confocal image of HeLa cell stained with Tuberin (TSC2) Antibody (S1798). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.
Tuberin / TSC2 Antibody - Western blot of TSC2-pS1798 in Ramos cell line lysates (35 ug/lane). TSC2 (arrow) was detected using the purified antibody.
Tuberin / TSC2 Antibody - Western blot of lysates from PC-3, LNCaP, HeLa cell line (from left to right), using Tuberin (TSC2) Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Tuberin / TSC2 Antibody - Western blot of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, HeLa, cell line (from left to right), using Tuberin (TSC2) Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Tuberin / TSC2 Antibody - Anti-TSC2 / Tuberin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Tuberin / TSC2 Antibody - Fluorescent confocal image of HeLa cell stained with Tuberin (TSC2) Antibody (S1798). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Tuberin (TSC2) primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Tuberin (TSC2) immunoreactivity is localized to Cytoplasm significantly.
Tuberin / TSC2 Antibody - Western blot of TSC2-pS1798 in Ramos cell line lysates (35 ug/lane). TSC2 (arrow) was detected using the purified antibody.
Tuberin / TSC2 Antibody - Western blot of lysates from PC-3, LNCaP, HeLa cell line (from left to right), using Tuberin (TSC2) Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Tuberin / TSC2 Antibody - Western blot of lysates from SH-SY5Y, MCF-7, PC-3, LNCaP, HeLa, cell line (from left to right), using Tuberin (TSC2) Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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IHCPlus™ Tuberin / TSC2 Antibody Ser1798 Polyclonal IF,IHC,WB LS‑B6311

IHCPlus™ Tuberin / TSC2 Antibody Ser1798 Polyclonal IF,IHC,WB LS‑B6311

Note: This antibody replaces LS-C100143
Antibody:
Rabbit Polyclonal to Human Tuberin / TSC2
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B6311-200
200 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Tuberin / TSC2
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TSC2 antibody LS-B6311 is an unconjugated rabbit polyclonal antibody to human TSC2 (Tuberin ). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Tuberin / TSC2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8213 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6311. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Ser1798
Specificity
This Tuberin (TSC2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1776-1805 amino acids from human Tuberin (TSC2).
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Tuberin / TSC2
P49815 NM_000548 NP_000539.2

Validation

Tuberin / TSC2 Antibody - Anti-TSC2 / Tuberin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TSC2 / Tuberin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TSC2 / Tuberin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 11/19/2019