TPPP | 25 kDa brain-specific protein | p25-alpha | p25 | p25alpha | p24 | TPPP1
(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Peptide with sequence C-DPSKDRAAKRLSLES, from the internal region of the protein sequence according to NP_008961.1.
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.00 - 0.01 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kD according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01 ug/ml. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.