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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2540-50 | 50 µg (1 mg/ml) | $515 |
![TGFB4 / LEFTY2 Antibody - Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-tgfb4-lefty2-antibody-clone-7c5g1h6h10-ls-b2540/136457_67032.jpg)
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IHC‑plus™ Monoclonal Mouse anti‑Human TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10, IHC, WB) LS‑B2540
IHC‑plus™ Monoclonal Mouse anti‑Human TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10, IHC, WB) LS‑B2540
Note: This antibody replaces LS-C18793
Antibody:
TGFB4 / LEFTY2 Mouse anti-Human Monoclonal (7C5G1H6H10) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
TGFB4 / LEFTY2 Mouse anti-Human Monoclonal (7C5G1H6H10) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
LEFTY2 antibody LS-B2540 is an unconjugated mouse monoclonal antibody to human LEFTY2 (TGFB4). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human TGFB4 / LEFTY2
Synonyms
LEFTY2 | EBAF | LEFTYA | Protein lefty-2 | LEFTA | TGFB4 | Protein lefty-A | TGF-beta-4
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
7C5G1H6H10
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Specificity
Reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:2000)
- ELISA (1:5000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2540 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2540 was determined to be 10 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TGFB4 / LEFTY2
Validation
Anti-LEFTY2 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LEFTY2 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) for IHC, WB/Western, ELISA LS-B2540 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1. Tsukui T, Capdevila J, Tamura K, Ruiz-Lozano P, Rodriguez-Esteban C, Yonei-Tamura S, Magalln J, Chandraratna RA, Chien K, Blumberg B, Evans RM, Belmonte JC. Proceedings of the National Academy of Sciences of the United States of America. 1999 96:11376-81.
Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor. Ulloa L, Tabibzadeh S. The Journal of biological chemistry. 2001 276:21397-404.
Customer Reviews (0)
Featured Products
Reactivity:
Human
Range:
0.78-50 ng/ml
Reactivity:
Human
Range:
Positive/Negative
Reactivity:
All species
Range:
7.8-500 pg/ml
Reactivity:
Bovine
Range:
12.5-800 pg/ml
Species:
Human
Applications:
ICC, Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/31/2024
Date Requested: 10/31/2024
