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Catalog Number Size Price
LS-B420-50 50 µg (1 mg/ml) $535 
SAE1 Antibody - Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SAE1 Antibody - Anti-SUMO Activating Enzyme (SAE1) Antibody - SDS-PAGE/Western Blot. Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kD band present in 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. Molecular weight markers are shown for both the Coomassie-stained gel and the western blot (lane M, red, 700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. SDS-PAGE image courtesy of Proteome Resources, Englewood, CO.
SAE1 Antibody - Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
SAE1 Antibody - Anti-SUMO Activating Enzyme (SAE1) Antibody - SDS-PAGE/Western Blot. Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kD band present in 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. Molecular weight markers are shown for both the Coomassie-stained gel and the western blot (lane M, red, 700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. SDS-PAGE image courtesy of Proteome Resources, Englewood, CO.
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IHC‑plus™ Polyclonal Rabbit anti‑Human SAE1 Antibody (IHC, IF, WB) LS‑B420

IHC‑plus™ Polyclonal Rabbit anti‑Human SAE1 Antibody (IHC, IF, WB) LS‑B420

Note: This antibody replaces LS-C19036, LS-C56910
Antibody:
SAE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$535
LS-B420-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SAE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SAE1 antibody LS-B420 is an unconjugated rabbit polyclonal antibody to human SAE1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SAE1
Synonyms
SAE1 | Activator of SUMO1 | AOS1 | UBLE1A | HSPC140 | SUA1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-SUMO Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B420 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B420 was determined to be 5 ug/ml.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SAE1
Q9UBE0 NM_005500 NP_005491.1

Validation

SAE1 Antibody - Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ SAE1 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B420 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/18/2024