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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SAE1

SUMO1 activating enzyme subunit 1

The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Gene Name: SUMO1 activating enzyme subunit 1
Synonyms: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1
Target Sequences: NM_005500 NP_005491.1 Q9UBE0

Filtered by: None
Target
SAE1 (57)
Reactivity
Human (57)
Mouse (29)
Rat (20)
Bat (1)
Bovine (12)
Chimpanzee (6)
Dog (12)
Guinea pig (4)
Horse (5)
Monkey (10)
Orangutan (6)
Pig (8)
Rabbit (9)
Xenopus (4)
Zebrafish (4)
Application
IHC (27)
IHC-P (10)
WB (53)
DB (1)
ELISA (24)
ICC (5)
IF (11)
IP (2)
Peptide-ELISA (2)
Host
rabbit (39)
mouse (16)
goat (1)
sheep (1)
Isotype
IgG (23)
IgG1 (8)
IgG1,l (1)
IgG2b (6)
Clonality
monoclonal mc (16)
polyclonal pc (41)
Clone
1G4-1G5 (1)
1G8 (2)
2946C4a (1)
OTI2B2 (2)
OTI3C11 (2)
OTI3C3 (2)
OTI6B11 (2)
OTI6C7 (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
Carrier-free (6)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (45)
Epitope
aa300-329 (8)
N-Terminus (4)
aa71-120 (4)
Internal (2)
190-270 aa, Internal (1)
Leu247 (1)
aa221-270 (1)
aa296-346 (1)
aa3-247 (1)
aa334-346 (1)
pSer185 (1)
Publications
No (57)
Showing 1-25 of 57 results

IHC-plus™ SAE1 Antibody (clone 1G8) LS-B9408
Mouse Monoclonal [clone 1G8] (IgG2b) to Human SAE1
Human
IF, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-SAE1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-SAE1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAE1.

IHC-plus™ SAE1 Antibody (clone 1G4-1G5) LS-B6203
Mouse Monoclonal [clone 1G4-1G5] (IgG1,l) to Human SAE1
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg  $495.00
Anti-SAE1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell. [antibody concentration 10 ug/ml]

IHC-plus™ SAE1 Antibody LS-B420
Rabbit Polyclonal to Human SAE1
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-SAE1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SUMO Activating Enzyme (SAE1) Antibody - SDS-PAGE/Western Blot. Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kD band present in 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu ( for 45 min at room temperature. Molecular weight markers are shown for both the Coomassie-stained gel and the western blot (lane M, red, 700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. SDS-PAGE image courtesy of Proteome Resources, Englewood, CO.

SAE1 Antibody (Internal) LS-C353229
Rabbit Polyclonal to Human SAE1
Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of SAE1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

SAE1 Antibody (aa3-247) LS-C186319
Rabbit Polyclonal to Human SAE1
Human
ICC, IHC, IHC-P, WB
Unconjugated
100µl 0.72mg/ml  $355.00
IHC of paraffin-embedded A549 Xenograft using SAE1 antibody at 1:100 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using SAE1 antibody at 1:200 dilution.

SAE1 Antibody (clone 1G8, Carrier-free) LS-C788012
Mouse Monoclonal [clone 1G8] (IgG2b) to Human SAE1
Human
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Anti-SAE1 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SAE1 mouse monoclonal antibody.

SAE1 Antibody LS-C753733
Rabbit Polyclonal (IgG) to Human SAE1
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $355.00

SAE1 Antibody (190-270 aa, Internal) LS-C385989
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of SAE1 antibody

SAE1 Antibody (clone OTI6C7) LS-C339476
Mouse Monoclonal [clone OTI6C7] (IgG1) to Human SAE1
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human pancreas tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

SAE1 Antibody (clone OTI6C7, Carrier-free) LS-C799542
Mouse Monoclonal [clone OTI6C7] (IgG1) to Human SAE1
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,

SAE1 Antibody (clone OTI6B11) LS-C339477
Mouse Monoclonal [clone OTI6B11] (IgG2b) to Human SAE1
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

SAE1 Antibody (clone OTI6B11, Carrier-free) LS-C798215
Mouse Monoclonal [clone OTI6B11] (IgG2b) to Human SAE1
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,

SAE1 Antibody (clone OTI3C3) LS-C339550
Mouse Monoclonal [clone OTI3C3] (IgG1) to Human SAE1
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human pancreas tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

SAE1 Antibody (clone OTI3C3, Carrier-free) LS-C790576
Mouse Monoclonal [clone OTI3C3] (IgG1) to Human SAE1
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,

SAE1 Antibody LS-C800385
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Immunofluorescence Image

SAE1 Antibody (Leu247) LS-C176333
Rabbit Polyclonal to Human SAE1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µl  $345.00
Western blot of AOS1 (L247) pAb in extracts from 293 cells.

SAE1 Antibody LS-C401311
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using SAE1 Polyclonal Antibody at dilution of 1:15.
Immunohistochemistry of paraffin-embedded Human cervical cancer using SAE1 Polyclonal Antibody at dilution of 1:15.

SAE1 Antibody LS-C404587
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.4mg/ml  $205.00; 60µl 0.4mg/ml  $235.00; 120µl 0.4mg/ml  $295.00; 200µl 0.4mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using SAE1 Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of paraffin-embedded Human cervical cancer using SAE1 Polyclonal Antibody at dilution of 1:20.

SAE1 Antibody (aa300-329) LS-C98475
Rabbit Polyclonal to Human SAE1
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The AOS1 C-term antibody is used in Western blot to detect AOS1 in mouse heart tissue lysate.

SAE1 Antibody LS-C415962
Rabbit Polyclonal to Human SAE1
Mouse, Rat, Human
ICC, IF, WB
Unconjugated
100µg  $305.00
Western blot of 293 cell lysate using AOS1 Antibody

SAE1 Antibody (aa300-329) LS-C328802
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

SAE1 Antibody (aa300-329, AP) LS-C206905
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

SAE1 Antibody (clone 2946C4a) LS-C184674
Mouse Monoclonal [clone 2946C4a] (IgG1) to Human SAE1
Human
DB, ICC, WB
Unconjugated
50µg  $325.00
Western blot of immunized recombinant protein using SAE1 antibody.

SAE1 Antibody LS-C676444
Rabbit Polyclonal (IgG) to Human SAE1
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $235.00; 100µl  $285.00
Immunohistochemistry of paraffin-embedded human brain tissue at dilution 1:100
Immunohistochemistry of paraffin-embedded human colon cancer at dilution 1:100

SAE1 Antibody (aa221-270) LS-C118599
Rabbit Polyclonal (IgG) to Human SAE1
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide.
Showing 1-25 of 57 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).