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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B419-50 50 µg $485 
RPS6KB1 / P70S6K / S6K Antibody - Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
RPS6KB1 / P70S6K / S6K Antibody - Anti-p70S6K antibody - Western Blot. Western blot of Affinity Purified anti-p70S6K antibody shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.
RPS6KB1 / P70S6K / S6K Antibody - Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
RPS6KB1 / P70S6K / S6K Antibody - Anti-p70S6K antibody - Western Blot. Western blot of Affinity Purified anti-p70S6K antibody shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.
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IHCPlus™ Polyclonal Rabbit anti‑Human RPS6KB1 / P70S6K / S6K Antibody (IHC, WB) LS‑B419

IHCPlus™ Polyclonal Rabbit anti‑Human RPS6KB1 / P70S6K / S6K Antibody (IHC, WB) LS‑B419

Note: This antibody replaces LS-C18886
Antibody:
Rabbit Polyclonal to Human RPS6KB1 / P70S6K / S6K
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B419-50
50 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human RPS6KB1 / P70S6K / S6K
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
S6K antibody LS-B419 is an unconjugated rabbit polyclonal antibody to human S6K (RPS6KB1 / P70S6K). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human RPS6KB1 / P70S6K / S6K
Synonyms
RPS6KB1 | 70 kDa S6 kinase | p70 S6KA | p70-alpha | p70-S6K | p70 S6 kinase alpha | p70s6 kinase | S6K-beta-1 | STK14A | p70 ribosomal S6 kinase | p70 S6 kinase, alpha 1 | p70 S6 kinase, alpha 2 | p70 S6K-alpha | p70-S6K 1 | p70S6K | p70S6K1 | PS6K | Ribosomal protein S6 kinase I | S6K | S6K1 | p70 ribosomal S6 kinase alpha | p70(S6K)-alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p70S6K protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with p70S6K proteins from human, rat and mouse. Reactivity against homologues from other sources is not known.
Usage
Immunohistochemistry: LS-B419 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B419 was determined to be 5 ug/ml.
Presentation
0.1 M Tris-Chloride, pH 8.0, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RPS6KB1 / P70S6K / S6K
P23443 NM_003161 NP_003152.1

Validation

RPS6KB1 / P70S6K / S6K Antibody - Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-RPS6KB1 / S6K antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ RPS6KB1 / P70S6K / S6K Antibody for IHC, WB/Western, IP, ELISA LS-B419 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/10/2020