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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B7101-0.05 0.05 mg (1 mg/ml) $425 
ROCK2 Antibody - Anti-ROCK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ROCK2 Antibody - Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 ug/ml.
ROCK2 Antibody - Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/ml
ROCK2 Antibody - Anti-ROCK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ROCK2 Antibody - Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 ug/ml.
ROCK2 Antibody - Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/ml
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IHCPlus™ Polyclonal Rabbit anti‑Human ROCK2 Antibody (IHC, IF, WB) LS‑B7101

IHCPlus™ Polyclonal Rabbit anti‑Human ROCK2 Antibody (IHC, IF, WB) LS‑B7101

Note: This antibody replaces LS-C144366
Antibody:
Rabbit Polyclonal to Human ROCK2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7101-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human ROCK2
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ROCK2 antibody LS-B7101 is an unconjugated rabbit polyclonal antibody to ROCK2 from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ROCK2
Synonyms
ROCK2 | KIAA0619 | p164 ROCK-2 | Rho kinase 2 | RhoA-associated kinase-2 | ROCK-II | Rho kinase II | Rok beta | Rok2 | RockII
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 15 AA synthetic peptide near the center of human ROCK2. The immunogen is located within aa 920-970 of ROCK2.
Blocking Peptide
LS-E31112 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B7101. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Usage
ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 153 kDa Observed: 160 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ROCK2
O75116 NM_004850 NP_004841.2

Validation

ROCK2 Antibody - Anti-ROCK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROCK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROCK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ROCK2 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B7101 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 5/31/2020