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Catalog Number Size Price
LS-B81-50 50 µg (1.1 mg/ml) $515 
ROBO1 Antibody - Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ROBO1 Antibody - Immunofluorescence - Anti-ROBO-1 Antibody. Staining of ROBO1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining. Image from Lindenmeyer MT et al. Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7): e11545, Fig 5.
ROBO1 Antibody - Immunofluorescence - Anti-ROBO-1 Antibody. 1/50 staining mouse lung tissue sections (adult, frozen 100mum whole mount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
ROBO1 Antibody - Western Blot - Anti-ROBO-1 Antibody. Western blot of Affinity Purified anti-ROBO-1 antibody shows detection of a band at ~181 kD corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
ROBO1 Antibody - Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ROBO1 Antibody - Immunofluorescence - Anti-ROBO-1 Antibody. Staining of ROBO1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining. Image from Lindenmeyer MT et al. Systematic Analysis of a Novel Human Renal Glomerulus-Enriched Gene Expression Dataset. PLoS One. 2010 July 12;5(7): e11545, Fig 5.
ROBO1 Antibody - Immunofluorescence - Anti-ROBO-1 Antibody. 1/50 staining mouse lung tissue sections (adult, frozen 100mum whole mount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
ROBO1 Antibody - Western Blot - Anti-ROBO-1 Antibody. Western blot of Affinity Purified anti-ROBO-1 antibody shows detection of a band at ~181 kD corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ROBO1 Antibody (aa1625‑1650, IHC, IF, WB) LS‑B81

IHC‑plus™ Polyclonal Rabbit anti‑Human ROBO1 Antibody (aa1625‑1650, IHC, IF, WB) LS‑B81

Note: This antibody replaces LS-C19082
Antibody:
ROBO1 Rabbit anti-Human Polyclonal (aa1625-1650) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B81-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ROBO1 Rabbit anti-Human Polyclonal (aa1625-1650) Antibody
Application:
IHC, IHC-P, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
ROBO1 antibody LS-B81 is an unconjugated rabbit polyclonal antibody to ROBO1 (aa1625-1650) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ROBO1
Synonyms
ROBO1 | Deleted in U twenty twenty | H-Robo-1 | Roundabout 1 | SAX3 | DUTT1 | Roundabout homolog 1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an C-Terminal region near amino acids 1625-1650 of Human ROBO-1.
Epitope
aa1625-1650
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and dog sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of ROBO-1. Cross reactivity with ROBO-1 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation
  • ELISA (1:30000 - 1:60000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B81 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B81 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ROBO1
Q9Y6N7 NM_002941 NP_002932.1

Validation

ROBO1 Antibody - Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ROBO1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ROBO1 Antibody (aa1625-1650) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B81 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/8/2024