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Catalog Number Size Price
LS-B45-50 50 µg (1 mg/ml) $485 
PPARD / PPAR Delta Antibody - Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PPARD / PPAR Delta Antibody - Anti-PPAR Delta Antibody - Western Blot. Affinity Purified Anti-PPAR delta [Rabbit] is shown to detect a predominant band at ~43 kD corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4C followed reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) ( for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
PPARD / PPAR Delta Antibody - Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PPARD / PPAR Delta Antibody - Anti-PPAR Delta Antibody - Western Blot. Affinity Purified Anti-PPAR delta [Rabbit] is shown to detect a predominant band at ~43 kD corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4C followed reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) ( for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Mouse PPARD / PPAR Delta Antibody (IHC, WB) LS‑B45

IHC‑plus™ Polyclonal Rabbit anti‑Mouse PPARD / PPAR Delta Antibody (IHC, WB) LS‑B45

Note: This antibody replaces LS-C18857, LS-C56907
Antibody:
PPARD / PPAR Delta Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B45-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PPARD / PPAR Delta Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PPAR Delta antibody LS-B45 is an unconjugated rabbit polyclonal antibody to PPAR Delta (PPARD) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Mouse PPARD / PPAR Delta
Synonyms
PPARD | FAAR | NUCI | PPAR-beta | PPAR-delta | NR1C2 | NUC1 | NUCII | Nuclear hormone receptor 1 | PPARB | PPAR Delta
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-PPAR delta antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:5000)
  • ELISA (1:30000 - 1:70000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B45 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B45 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPARD / PPAR Delta
Q03181 NM_006238 NP_006229.1

Validation

PPARD / PPAR Delta Antibody - Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PPAR Delta antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ PPARD / PPAR Delta Antibody for IHC, WB/Western, ELISA LS-B45 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus. Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X. PloS one. 2012 7:e36504. (IHC-P; Mouse)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/8/2022