Target
Human ITGAL / CD11a
Synonyms
ITGAL | CD11A | Integrin alpha-L | LFA-1 alpha | LFA-1A | Integrin gene promoter | Alpha polypeptide) | CD11a antigen | LFA-1 | LFA1A
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-YQVRIQPSIHDHN, from the internal region of the protein sequence according to NP_002200.2NP_001107852.1.
Specificity
Human ITGAL / CD11a. This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee