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Catalog Number Size Price
LS-B7435-50 50 µg $485 
IL1RN Antibody - Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL1RN Antibody - Western Blot (reducing) of IL1RN / IL-1RA antibody
IL1RN Antibody - Western Blot (non-reducing) of IL1RN / IL-1RA antibody
IL1RN Antibody - Sandwich ELISA of IL1RN / IL-1RA antibody\
IL1RN Antibody - Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IL1RN Antibody - Western Blot (reducing) of IL1RN / IL-1RA antibody
IL1RN Antibody - Western Blot (non-reducing) of IL1RN / IL-1RA antibody
IL1RN Antibody - Sandwich ELISA of IL1RN / IL-1RA antibody\
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IHC‑plus™ Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑B7435

IHC‑plus™ Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑B7435

Note: This antibody replaces LS-C104493, LS-C188017
Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B7435-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
IL1RN antibody LS-B7435 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IL1RN
Synonyms
IL1RN | DIRA | IL-1ra | IL-1RN | IL1F3 | IL1RA | MVCD4 | IL1 inhibitor | IL1RN (IL1F3) | IRAP | ICIL-1RA | IL-1ra3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human IL-1RA.
Specificity
Human IL-1RA
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B7435 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-1RA (50 ng/ml) and Human IL-1alpha (50 pg/ml), a concentration of 2.5-3.7 ug/ml of this antibody is required. ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (LS-C104474) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1RN
P18510 NM_000577 NP_000568.1

Validation

IL1RN Antibody - Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ IL1RN Antibody for IHC, WB/Western, ELISA LS-B7435 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/8/2024